Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis
Anadara kagoshimensis (Tokunaga, 1906) is an invasive bivalve mollusk colonizing the Black Sea and Azov Sea. The content of 16 proteinogenic amino acids was determined in their hemolymph and soft tissue protein hydrolysates via ion-exchange chromatography followed by ninhydrin detection. High histid...
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Maik Nauka-Interperiodica Publishing
2024
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ir-123456789-287642024-08-16T11:29:06Z Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis Budkevich, E. V. Будкевич, Е. В. Anadara kagoshimensis Black Sea Alien species Hypoxia Protein hydrolysate Amino acid metabolism Sea of Azov Anadara kagoshimensis (Tokunaga, 1906) is an invasive bivalve mollusk colonizing the Black Sea and Azov Sea. The content of 16 proteinogenic amino acids was determined in their hemolymph and soft tissue protein hydrolysates via ion-exchange chromatography followed by ninhydrin detection. High histidine and proline levels were detected both in hemolymph and in soft tissue hydrolysates. Experimental hypoxia caused qualitative and quantitative changes in the content of free amino acids, namely the pool of aliphatic amino acids decreased 2-fold, while the pool of aromatic amino acids conversely increased. It is assumed that the metabolism of A. kagoshimensis shifts under hypoxic conditions toward anaerobic catabolism of amino acids and proteins as a source of substrates for the tricarboxylic acid and ornithine cycles. This leads to a significant accumulation of arginine, which is an allosteric activator of ornithine cycle reactions, and urea, which is a low-molecular-weight (LMW) antioxidant. Thereby, an LMW component of the antioxidant defense system forms in A. kagoshimensis, as manifested in high levels of such free radical scavengers as histidine and urea, which may contribute to the invasion success of this alien bivalve species in the Black and Azov Seas. 2024-08-16T11:09:54Z 2024-08-16T11:09:54Z 2024 Статья Golub, NA; Soldatov, AA; Ryabushko, VI; Kuznetsov, AV; Kurchenko, VP; Budkevich, EV. Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis // JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY. - 2024. - 60(1). - рр. 136-150. - DOI: 10.1134/S0022093024010101 https://dspace.ncfu.ru/handle/123456789/28764 en JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY application/pdf Maik Nauka-Interperiodica Publishing |
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| language |
English |
| topic |
Anadara kagoshimensis Black Sea Alien species Hypoxia Protein hydrolysate Amino acid metabolism Sea of Azov |
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Anadara kagoshimensis Black Sea Alien species Hypoxia Protein hydrolysate Amino acid metabolism Sea of Azov Budkevich, E. V. Будкевич, Е. В. Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis |
| description |
Anadara kagoshimensis (Tokunaga, 1906) is an invasive bivalve mollusk colonizing the Black Sea and Azov Sea. The content of 16 proteinogenic amino acids was determined in their hemolymph and soft tissue protein hydrolysates via ion-exchange chromatography followed by ninhydrin detection. High histidine and proline levels were detected both in hemolymph and in soft tissue hydrolysates. Experimental hypoxia caused qualitative and quantitative changes in the content of free amino acids, namely the pool of aliphatic amino acids decreased 2-fold, while the pool of aromatic amino acids conversely increased. It is assumed that the metabolism of A. kagoshimensis shifts under hypoxic conditions toward anaerobic catabolism of amino acids and proteins as a source of substrates for the tricarboxylic acid and ornithine cycles. This leads to a significant accumulation of arginine, which is an allosteric activator of ornithine cycle reactions, and urea, which is a low-molecular-weight (LMW) antioxidant. Thereby, an LMW component of the antioxidant defense system forms in A. kagoshimensis, as manifested in high levels of such free radical scavengers as histidine and urea, which may contribute to the invasion success of this alien bivalve species in the Black and Azov Seas. |
| format |
Статья |
| author |
Budkevich, E. V. Будкевич, Е. В. |
| author_facet |
Budkevich, E. V. Будкевич, Е. В. |
| author_sort |
Budkevich, E. V. |
| title |
Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis |
| title_short |
Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis |
| title_full |
Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis |
| title_fullStr |
Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis |
| title_full_unstemmed |
Effect of Hypoxia on Amino Acid Content in Hemolymph and Protein Hydrolysate of the Bivalve Mollusk Anadara kagoshimensis |
| title_sort |
effect of hypoxia on amino acid content in hemolymph and protein hydrolysate of the bivalve mollusk anadara kagoshimensis |
| publisher |
Maik Nauka-Interperiodica Publishing |
| publishDate |
2024 |
| url |
https://dspace.ncfu.ru/handle/123456789/28764 |
| work_keys_str_mv |
AT budkevichev effectofhypoxiaonaminoacidcontentinhemolymphandproteinhydrolysateofthebivalvemolluskanadarakagoshimensis AT budkevičev effectofhypoxiaonaminoacidcontentinhemolymphandproteinhydrolysateofthebivalvemolluskanadarakagoshimensis |
| _version_ |
1809808910218952704 |