Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry

A new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of d...

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Главные авторы:	Dotsenko, V. V., Доценко, В. В.
Formato:	Статья
Idioma:	English
Publicado em:	Elsevier B.V. 2024
Assuntos:	Derivatization UHPLC-MS/MS HRMS Non-targeted screening Phthalylglycyl chloride Sample preparation SPE
Acesso em linha:	https://dspace.ncfu.ru/handle/123456789/29144
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spelling	ir-123456789-291442024-10-17T08:21:32Z Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry Dotsenko, V. V. Доценко, В. В. Derivatization UHPLC-MS/MS HRMS Non-targeted screening Phthalylglycyl chloride Sample preparation SPE A new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed. 2024-10-17T08:20:47Z 2024-10-17T08:20:47Z 2024 Статья Temerdashev, A., Nesterenko, P.N., Atapattu, S.N., Feng, Y.-Q., Zorina, M., Zhurkina, K., Gashimova, E., Steshin, M.O., Dotsenko, V.V. Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry // Journal of Chromatography Open. - 2024. - 6. - статья № 100162. - DOI: 10.1016/j.jcoa.2024.100162 https://dspace.ncfu.ru/handle/123456789/29144 en Journal of Chromatography Open application/pdf Elsevier B.V.
institution	СКФУ
collection	Репозиторий
language	English
topic	Derivatization UHPLC-MS/MS HRMS Non-targeted screening Phthalylglycyl chloride Sample preparation SPE
spellingShingle	Derivatization UHPLC-MS/MS HRMS Non-targeted screening Phthalylglycyl chloride Sample preparation SPE Dotsenko, V. V. Доценко, В. В. Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
description	A new derivatization reagent, phthalylglycyl chloride (PG-Cl), for the analysis of urinary amino acids was demonstrated using reversed-phase ultra-high performance liquid chromatography coupled to electrospray ionization-quadrupole-time-of-flight mass spectrometer. The study compares the limits of detection and quantification for different sample preparation techniques with the commonly used and effective method of derivatization using dansyl chloride. The stability of the derivatives was investigated, and the study includes an example of urinary amino acid analysis using PG-Cl derivatization. The optimal values for pH, temperature, and concentration of the derivatization reagent were established. Limits of quantification in the range of 0.5–500 µg/mL were obtained for different amino acids. The possibility of phthalylglycyl chloride usage for non-target screening applications and targeted analysis of amino acids in urine was discussed. An advantages and issues of PG-Cl in comparison with commonly used acyl-chlorides (FMOC-Cl and DNS-Cl) provided and discussed.
format	Статья
author	Dotsenko, V. V. Доценко, В. В.
author_facet	Dotsenko, V. V. Доценко, В. В.
author_sort	Dotsenko, V. V.
title	Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
title_short	Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
title_full	Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
title_fullStr	Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
title_full_unstemmed	Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
title_sort	phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
publisher	Elsevier B.V.
publishDate	2024
url	https://dspace.ncfu.ru/handle/123456789/29144
work_keys_str_mv	AT dotsenkovv phthalylglycylchlorideasaderivatizationreagentfordeterminationofurinaryaminoacidsusingultrahighperformanceliquidchromatographycoupledwithhighresolutionmassspectrometry AT docenkovv phthalylglycylchlorideasaderivatizationreagentfordeterminationofurinaryaminoacidsusingultrahighperformanceliquidchromatographycoupledwithhighresolutionmassspectrometry
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Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry

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