GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING

For the mass use of genotyping by sequencing in sheep of the Russian Meat Merino breed, it is necessary to determine the loci of the genome with a sufficient frequency of occurrence in the population. To identify them, genotyping of Russian Meat Merino sheep was carried out using Ovine Infinium HD B...

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Главные авторы:	Krivoruchko, A. Y., Криворучко, А. Ю., Likhovid, A. A., Лиховид, А. А.
Format:	Статья
Sprog:	English
Udgivet:	Serbian Genetics Society 2024
Fag:	Breeding Russian Meat merino Genotyping by sequencing NGS Sheep SNP
Online adgang:	https://dspace.ncfu.ru/handle/123456789/29332
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id	ir-123456789-29332
record_format	dspace
spelling	ir-123456789-293322024-12-06T12:35:37Z GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING PRETRAGA POLIMORFIZMA ŠIROM GENOMA KORIŠĆENJEM ILUMINA ČIPA U RUSKIM MESNATIM MERINO OVCAMA ZA BUDUĆU GENOTIPIZACIJU SEKVENCIONIRANJEM Krivoruchko, A. Y. Криворучко, А. Ю. Likhovid, A. A. Лиховид, А. А. Breeding Russian Meat merino Genotyping by sequencing NGS Sheep SNP For the mass use of genotyping by sequencing in sheep of the Russian Meat Merino breed, it is necessary to determine the loci of the genome with a sufficient frequency of occurrence in the population. To identify them, genotyping of Russian Meat Merino sheep was carried out using Ovine Infinium HD BeadChip 600K. As a result of polymorphism evaluation of 606,000 loci, 555 SNPs were selected with a frequency of occurrence of both homozygous variants in the range of 0.2850-0.3149. After excluding substitutions located closer than 1cM, a list of 387 polymorphisms was obtained. The selected substitutions were located on all 26 autosomes. The greatest number of polymorphisms were on the 1, 3, 6, 9, 12 and 22 chromosomes. The least substitutions were found on chromosomes 4, 8, 11 and 19. Only one substitution with the required frequency of occurrence was identified on the X chromosome. The average distance between SNPs was 4,000 to 7,000 kbp. The list of polymorphisms we have chosen can be used to confirm the reliability of the origin in the molecular genetic examination of sheep of the Russian Meat Merino breed. 2024-12-06T12:32:12Z 2024-12-06T12:32:12Z 2024 Статья Krivoruchko, A., Likhovid, A., Kanibolotskaya, A., Krivoruchko, O., Skorykh, L., Kizilova, N., Yatsyk, O. GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING // Genetika. - 2024. - 56 (2). - pp. 357-368. - DOI: 10.2298/GENSR2402357K https://dspace.ncfu.ru/handle/123456789/29332 en Genetika application/pdf Serbian Genetics Society
institution	СКФУ
collection	Репозиторий
language	English
topic	Breeding Russian Meat merino Genotyping by sequencing NGS Sheep SNP
spellingShingle	Breeding Russian Meat merino Genotyping by sequencing NGS Sheep SNP Krivoruchko, A. Y. Криворучко, А. Ю. Likhovid, A. A. Лиховид, А. А. GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
description	For the mass use of genotyping by sequencing in sheep of the Russian Meat Merino breed, it is necessary to determine the loci of the genome with a sufficient frequency of occurrence in the population. To identify them, genotyping of Russian Meat Merino sheep was carried out using Ovine Infinium HD BeadChip 600K. As a result of polymorphism evaluation of 606,000 loci, 555 SNPs were selected with a frequency of occurrence of both homozygous variants in the range of 0.2850-0.3149. After excluding substitutions located closer than 1cM, a list of 387 polymorphisms was obtained. The selected substitutions were located on all 26 autosomes. The greatest number of polymorphisms were on the 1, 3, 6, 9, 12 and 22 chromosomes. The least substitutions were found on chromosomes 4, 8, 11 and 19. Only one substitution with the required frequency of occurrence was identified on the X chromosome. The average distance between SNPs was 4,000 to 7,000 kbp. The list of polymorphisms we have chosen can be used to confirm the reliability of the origin in the molecular genetic examination of sheep of the Russian Meat Merino breed.
format	Статья
author	Krivoruchko, A. Y. Криворучко, А. Ю. Likhovid, A. A. Лиховид, А. А.
author_facet	Krivoruchko, A. Y. Криворучко, А. Ю. Likhovid, A. A. Лиховид, А. А.
author_sort	Krivoruchko, A. Y.
title	GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
title_short	GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
title_full	GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
title_fullStr	GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
title_full_unstemmed	GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING
title_sort	genome-wide search polymorphisms using illumina beadchip in russian meat merino sheep for future genotyping by sequencing
publisher	Serbian Genetics Society
publishDate	2024
url	https://dspace.ncfu.ru/handle/123456789/29332
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GENOME-WIDE SEARCH POLYMORPHISMS USING ILLUMINA BEADCHIP IN RUSSIAN MEAT MERINO SHEEP FOR FUTURE GENOTYPING BY SEQUENCING

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