MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE

Cryoprotectants enable the long-term storage of biomaterials. Despite progress in cryopreservation, there are a number of problems associated with damage to the cell membranes that result from insufficient efficacy and toxicity of some components. In this regard, it is important to develop non-toxic...

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Главные авторы:	Vlasov, A. A., Власов, А. А., Andrusenko, S. F., Андрусенко, С. Ф., Denisova, E. V., Денисова, Е. В., Elkanova, A. B., Эльканова, А. Б., Kadanova, A. A., Каданова, А. А., Melchenko, E. A., Мельченко, Е. А., Sokulskaya, N. N., Сокульская, Н. Н.
Формат:	Статья
Язык:	English
Опубликовано:	Pirogov Russian National Research Medical University 2024
Темы:	Cryopreservation Platelets Erythrocytes Lactulose Leukocytes Moderate low temperature
Online-ссылка:	https://dspace.ncfu.ru/handle/123456789/29338
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id	ir-123456789-29338
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spelling	ir-123456789-293382024-12-09T12:43:22Z MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE МОРФОФУНКЦИОНАЛЬНОЕ СОСТОЯНИЕ КРИОКОНСЕРВИРОВАННЫХ ФОРМЕННЫХ ЭЛЕМЕНТОВ КРОВИ ПРИ УМЕРЕННО НИЗКИХ ТЕМПЕРАТУРАХ Vlasov, A. A. Власов, А. А. Andrusenko, S. F. Андрусенко, С. Ф. Denisova, E. V. Денисова, Е. В. Elkanova, A. B. Эльканова, А. Б. Kadanova, A. A. Каданова, А. А. Melchenko, E. A. Мельченко, Е. А. Sokulskaya, N. N. Сокульская, Н. Н. Cryopreservation Platelets Erythrocytes Lactulose Leukocytes Moderate low temperature Cryoprotectants enable the long-term storage of biomaterials. Despite progress in cryopreservation, there are a number of problems associated with damage to the cell membranes that result from insufficient efficacy and toxicity of some components. In this regard, it is important to develop non-toxic cryopreservation agents performing well at low temperature. The study was aimed to assess morphofunctional features of blood cells in the lactulose-based cryopreservation agent considering the effects of moderate low temperature (–40 °С). Blood cells (leukocytes, erythrocytes, platelets) collected from 30 conditionally healthy female voluntary donors aged 18–23 years were assessed. The complete blood count test was performed using the Gemalight 1270 automated hematology analyzer. Computerized cytomorphometric assessement was performed using the MECOS-C2 hardware and software complex. The study results showed morphological and functional integrity of blood cells after the 24 h storage at the temperature of–40 °С when added the lactulose-based cryopreservation agent developed: erythrocytes — 85.3 ± 0.30% (p < 0.05), platelets – 75 ± 0.71% (p < 0.05), leukocytes – 90.1 ± 0.91% (p < 0.05) of the values reported before freezing. The findings demonstrate the potential of using lactulose as a non-toxic component of cryopreservation systems, which will expand the range of cryopreservation agents used and make it possible to analyze morphofunctional parameters of frozen whole blood samples when conducting large-scale studies. 2024-12-09T12:41:25Z 2024-12-09T12:41:25Z 2024 Статья Vlasov, A.A., Andrusenko, S.F., Denisova, E.V., Elkanova, A.B., Kadanova, A.A., Melchenko, E.A., Sokulskaya, N.N., Domenyuk, D.A. MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE // Bulletin of Russian State Medical University. - 2024. - (5). - pp. 72-78. - DOI: 10.24075/brsmu.2024.038 https://dspace.ncfu.ru/handle/123456789/29338 en Bulletin of Russian State Medical University application/pdf application/pdf Pirogov Russian National Research Medical University
institution	СКФУ
collection	Репозиторий
language	English
topic	Cryopreservation Platelets Erythrocytes Lactulose Leukocytes Moderate low temperature
spellingShingle	Cryopreservation Platelets Erythrocytes Lactulose Leukocytes Moderate low temperature Vlasov, A. A. Власов, А. А. Andrusenko, S. F. Андрусенко, С. Ф. Denisova, E. V. Денисова, Е. В. Elkanova, A. B. Эльканова, А. Б. Kadanova, A. A. Каданова, А. А. Melchenko, E. A. Мельченко, Е. А. Sokulskaya, N. N. Сокульская, Н. Н. MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE
description	Cryoprotectants enable the long-term storage of biomaterials. Despite progress in cryopreservation, there are a number of problems associated with damage to the cell membranes that result from insufficient efficacy and toxicity of some components. In this regard, it is important to develop non-toxic cryopreservation agents performing well at low temperature. The study was aimed to assess morphofunctional features of blood cells in the lactulose-based cryopreservation agent considering the effects of moderate low temperature (–40 °С). Blood cells (leukocytes, erythrocytes, platelets) collected from 30 conditionally healthy female voluntary donors aged 18–23 years were assessed. The complete blood count test was performed using the Gemalight 1270 automated hematology analyzer. Computerized cytomorphometric assessement was performed using the MECOS-C2 hardware and software complex. The study results showed morphological and functional integrity of blood cells after the 24 h storage at the temperature of–40 °С when added the lactulose-based cryopreservation agent developed: erythrocytes — 85.3 ± 0.30% (p < 0.05), platelets – 75 ± 0.71% (p < 0.05), leukocytes – 90.1 ± 0.91% (p < 0.05) of the values reported before freezing. The findings demonstrate the potential of using lactulose as a non-toxic component of cryopreservation systems, which will expand the range of cryopreservation agents used and make it possible to analyze morphofunctional parameters of frozen whole blood samples when conducting large-scale studies.
format	Статья
author	Vlasov, A. A. Власов, А. А. Andrusenko, S. F. Андрусенко, С. Ф. Denisova, E. V. Денисова, Е. В. Elkanova, A. B. Эльканова, А. Б. Kadanova, A. A. Каданова, А. А. Melchenko, E. A. Мельченко, Е. А. Sokulskaya, N. N. Сокульская, Н. Н.
author_facet	Vlasov, A. A. Власов, А. А. Andrusenko, S. F. Андрусенко, С. Ф. Denisova, E. V. Денисова, Е. В. Elkanova, A. B. Эльканова, А. Б. Kadanova, A. A. Каданова, А. А. Melchenko, E. A. Мельченко, Е. А. Sokulskaya, N. N. Сокульская, Н. Н.
author_sort	Vlasov, A. A.
title	MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE
title_short	MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE
title_full	MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE
title_fullStr	MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE
title_full_unstemmed	MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE
title_sort	morphofunctional state of cryopreserved blood cells at moderate low temperature
publisher	Pirogov Russian National Research Medical University
publishDate	2024
url	https://dspace.ncfu.ru/handle/123456789/29338
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MORPHOFUNCTIONAL STATE OF CRYOPRESERVED BLOOD CELLS AT MODERATE LOW TEMPERATURE

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