Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...

Disgrifiad llawn

Wedi'i Gadw mewn:

Manylion Llyfryddiaeth
Prif Awduron:	Povetkin, S. N., Поветкин, С. Н.
Fformat:	Статья
Iaith:	English
Cyhoeddwyd:	Academic Press Inc. 2025
Pynciau:	DNA melting temperature Thermodynamics of PCR PCR optimization PCR efficiency Magnesium chloride (MgCl<sub>2</sub>)
Mynediad Ar-lein:	https://dspace.ncfu.ru/handle/123456789/30526
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

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