Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...

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Bibliografiske detaljer
Главные авторы:	Povetkin, S. N., Поветкин, С. Н.
Format:	Статья
Sprog:	English
Udgivet:	Academic Press Inc. 2025
Fag:	DNA melting temperature Thermodynamics of PCR PCR optimization PCR efficiency Magnesium chloride (MgCl<sub>2</sub>)
Online adgang:	https://dspace.ncfu.ru/handle/123456789/30526
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!

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