Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity
Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...
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| Main Authors: | , |
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| Format: | Статья |
| Language: | English |
| Published: |
Academic Press Inc.
2025
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| Subjects: | |
| Online Access: | https://dspace.ncfu.ru/handle/123456789/30526 |
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