Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...

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Bibliografski detalji
Glavni autori:	Povetkin, S. N., Поветкин, С. Н.
Format:	Статья
Jezik:	English
Izdano:	Academic Press Inc. 2025
Teme:	DNA melting temperature Thermodynamics of PCR PCR optimization PCR efficiency Magnesium chloride (MgCl<sub>2</sub>)
Online pristup:	https://dspace.ncfu.ru/handle/123456789/30526
Oznake:	Dodaj oznaku Bez oznaka, Budi prvi tko označuje ovaj zapis!

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