Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...

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書誌詳細
主要な著者:	Povetkin, S. N., Поветкин, С. Н.
フォーマット:	Статья
言語:	English
出版事項:	Academic Press Inc. 2025
主題:	DNA melting temperature Thermodynamics of PCR PCR optimization PCR efficiency Magnesium chloride (MgCl<sub>2</sub>)
オンライン･アクセス:	https://dspace.ncfu.ru/handle/123456789/30526
タグ:	タグ追加タグなし, このレコードへの初めてのタグを付けませんか!

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