Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...

Volledige beschrijving

Bewaard in:

Bibliografische gegevens
Hoofdauteurs:	Povetkin, S. N., Поветкин, С. Н.
Formaat:	Статья
Taal:	English
Gepubliceerd in:	Academic Press Inc. 2025
Onderwerpen:	DNA melting temperature Thermodynamics of PCR PCR optimization PCR efficiency Magnesium chloride (MgCl<sub>2</sub>)
Online toegang:	https://dspace.ncfu.ru/handle/123456789/30526
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!

Gelijkaardige items

IR luminescence of CaGa2O4: Yb3+ excited by 940 and 980 nm radiation
door: Mar’ina, U. A., et al.
Gepubliceerd in: (2024)

Hydrophobization of Silicon Dioxide Microspheres With Resin Acids of Natural Gums
door: Filippov, D. D., et al.
Gepubliceerd in: (2025)

Investigation of the Effect of the Type of Precursor and Precipitator on the Structure of Nanoscale Magnesium Carbonate
door: Blinov, A. V., et al.
Gepubliceerd in: (2025)

Development of a Method for Obtaining Nanoscale Magnesium Carbonate Stabilized with Chitosan as the Basis of Scaffold Matrices for Regenerative Medicine
door: Blinov, A. V., et al.
Gepubliceerd in: (2025)

Synthesis and investigation of nanoscale magnesium carbonate stabilized with hyaluronic acid
door: Blinov, A. V., et al.
Gepubliceerd in: (2025)

Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
door: Dotsenko, V. V., et al.
Gepubliceerd in: (2023)

Физика (in English) учебник для вузов
door: Лебедев Д. В.
Gepubliceerd in: (2023)

Phthalylglycyl chloride as a derivatization reagent for determination of urinary amino acids using ultra high-performance liquid chromatography coupled with high resolution mass spectrometry
door: Dotsenko, V. V., et al.
Gepubliceerd in: (2024)

Oxidative coupling of tetraalkynyltin with aldehydes leading to alkynyl ketones
door: Aksenov, N. A., et al.
Gepubliceerd in: (2018)

Influence of MgO and CaO sintering additives on thermophysical, luminescent and optical properties of LuAG: Yb3+ laser ceramics
door: Lapin, V. A., et al.
Gepubliceerd in: (2024)

Structure and properties of europium (III) triaquatriglycinate chloride
door: Aksenov, N. A., et al.
Gepubliceerd in: (2020)

The Comprehension Of Private Copper
door: Joseph R. K.
Gepubliceerd in: (2013)

Reading Comprehension in Law учебник
door: Алисевич, М. В.

Reading Comprehension in Law учебник для спо
door: Алисевич, М. В.

Reading and Comprehension учебно-методическое пособие по практике английского языка
door: Малахова, С. А.

Business English reading comprehension сборник текстов для чтения и аннотирования по деловому английскому языку «business english reading comprehension» для студентов всех специальностей экономической направленности
door: Моисеева Ф. А.
Gepubliceerd in: (2023)

Comprehensive geriatric assessment: from theory to practice study aid
door: Bulgakova, S. V.

A way to successful reading comprehension учебно-методическое пособие для аспирантов
door: Кондратьева И. И.
Gepubliceerd in: (2011)

Intercultural patchwork (Listening comprehension skills development) учебно-методическое пособие
door: Жерновая О. Р.
Gepubliceerd in: (2021)

Unravelling the potential of insects for medicinal purposes – A comprehensive review
door: Blinov, A. V., et al.
Gepubliceerd in: (2023)

A Comprehensive Grammar of Contemporary English in Higher Education Studies Учебник
door: Андреева Е.Ю

Development of a Comprehensive Technology for Analyzing Data on the Used Car Market
door: Lapina, M. A., et al.
Gepubliceerd in: (2025)

Improve Your English Skills: Reading Comprehension, Vocabulary and Grammar Practice: практикум
door: Пушкина Е. Н.
Gepubliceerd in: (2023)

World trade: reading comprehension. Формирование навыков работы с профессиональными текстами
door: Душинина Е. В.
Gepubliceerd in: (2023)

SYNTHESIS OF LOW-AGGLOMERATED YAG:Yb NANOPOWDERS FOR TRANSPARENT CERAMICS BY METHOD OF REVERSE CO-PRECIPITATION FROM CHLORIDE SALTS
door: Nikova, M. S., et al.
Gepubliceerd in: (2024)

Перевод медицинского текста учебное пособие для подготовки к практическим занятиям по дисциплине «иностранный язык»
door: Ромашкина С. В.
Gepubliceerd in: (2024)

Determination of the Concentration and Accumulation Time of Triplet Molecules in Frozen Solutions from Change in Phosphorescence Intensity
door: Erina, M. V., et al.
Gepubliceerd in: (2023)

Desymmetrization of cyclopropenes via the potassium-templated diastereoselective 7- exo- trig cycloaddition of tethered amino alcohols toward enantiopure cyclopropane-fused oxazepanones with antimycobacterial activity
door: Aksenov, N. A., et al.
Gepubliceerd in: (2018)

Glutaraldehyde cross-linking for improving the techno-functional properties of biopolymeric food packaging films; a comprehensive review
door: Nagdalian, A. A., et al.
Gepubliceerd in: (2025)

Advancements in Sybil Attack Detection: A Comprehensive Survey of Machine Learning-Based Approaches in Wireless Sensor Networks
door: Lapina, M. A., et al.
Gepubliceerd in: (2024)

Comprehensive Study of the Single High-Energy Laser Pulse Effect on the Wear Resistance of R6M5 Steel
door: Pinakhin, I. A., et al.
Gepubliceerd in: (2025)

Nano-priming of pea (Pisum sativum L.) seeds with CuO nanoparticles: Synthesis, stabilization, modeling, characterization, and comprehensive effect on germination and seedling parameters
door: Nagdalian, A. A., et al.
Gepubliceerd in: (2025)

On that specific Pillow
door: Emily E. D.
Gepubliceerd in: (2013)

Интеграция цифровых технологий в преподавание английского языка для специальных целей: подробное руководство. Integrating Digital Technologies into ESP Instruction: A Comprehensive Manual for Teachers учебное пособие для вузов
door: Соболева А. В.
Gepubliceerd in: (2025)

Comprehension Reading for the Students of Geography and Geology
door: Васильева М. А.

Effect of Scandium Concentration in the Y2O3–Sc2O3–Al2O3–Er2O3 Oxide Composition on the Thermophysical Properties of Optical Ceramics
door: Chikulina, I. S., et al.
Gepubliceerd in: (2025)

English for specific purposes учебное пособие
door: Карпова И. Г.
Gepubliceerd in: (2023)

English for Specific Purposes: Aviation учебное пособие
door: Лебедева Н. А.
Gepubliceerd in: (2023)

Cadastral Engineering. English for Specific Purposes учебное пособие

Lexico-grammatical specifics of the nominations of fantastic birds in Russian linguaculture
door: Gryaznova, V. M., et al.
Gepubliceerd in: (2024)