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Comprehensive review and meta-analysis of magnesium chloride optimization in PCR: Investigating concentration effects on reaction efficiency and template specificity

Optimizing the polymerase chain reaction (PCR) continues to be a major challenge in molecular biology, and obtaining the correct magnesium chloride (MgCl2) concentration is key to a successful reaction. A clear understanding of how MgCl2 affects PCR thermodynamics and kinetics is crucial for creatin...

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Главные авторы: Povetkin, S. N., Поветкин, С. Н.
Format: Статья
Jezik:English
Izdano: Academic Press Inc. 2025
Teme:
Online dostop:https://dspace.ncfu.ru/handle/123456789/30526
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