Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions

Adverse environmental conditions are severely limiting the use of microorganisms in food systems, such as probiotic delivery, where low pH causes a rapid decrease in the survival of ingested bacteria, and mixed-culture fermentation, where stepwise changes and/or metabolites of individual microbial g...

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Главные авторы:	Neresyan, T., Нересян, Т., Gatina, Y. S., Гатина, Ю. С., Kulikova, I. K., Куликова, И. К., Lodygin, A. D., Лодыгин, А. Д.
Формат:	Статья
Язык:	English
Опубликовано:	MDPI 2022
Темы:	Dairy products Encapsulation Hydrogels Lactic acid bacteria Lactose-fermenting yeasts Polysaccharides Probiotics
Online-ссылка:	https://dspace.ncfu.ru/handle/20.500.12258/18563
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id	ir-20.500.12258-18563
record_format	dspace
spelling	ir-20.500.12258-185632025-03-06T12:23:43Z Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions Neresyan, T. Нересян, Т. Gatina, Y. S. Гатина, Ю. С. Kulikova, I. K. Куликова, И. К. Lodygin, A. D. Лодыгин, А. Д. Dairy products Encapsulation Hydrogels Lactic acid bacteria Lactose-fermenting yeasts Polysaccharides Probiotics Adverse environmental conditions are severely limiting the use of microorganisms in food systems, such as probiotic delivery, where low pH causes a rapid decrease in the survival of ingested bacteria, and mixed-culture fermentation, where stepwise changes and/or metabolites of individual microbial groups can hinder overall growth and production. In our study, model probiotic lactic acid bacteria (L. plantarum ATCC 8014, L. rhamnosus GG) and yeasts native to dairy mixed cultures (K. marxianus ZIM 1868) were entrapped in an optimized (cell, alginate and hardening solution concentration, electrostatic working parameters) Ca-alginate system. Encapsulated cultures were examined for short-term survival in the absence of nutrients (lactic acid bacteria) and longterm performance in acidified conditions (yeasts). In particular, the use of encapsulated yeasts in these conditions has not been previously examined. Electrostatic manufacturing allowed for the preparation of well-defined alginate microbeads (180–260 µm diameter), high cell-entrapment (95%) and viability (90%), and uniform distribution of the encapsulated cells throughout the hydrogel matrix. The entrapped L. plantarum maintained improved viabilities during 180 min at pH 2.0 (19% higher when compared to the free culture), whereas, L. rhamnosus appeared to be less robust. The encapsulated K. marxianus exhibited double product yields in lactose-and lactic acid-modified MRS growth media (compared to an unfavorable growth environment for freely suspended cells). Even within a conventional encapsulation system, the pH responsive features of alginate provided superior protection and production of encapsulated yeasts, allowing several applications in lacto-fermented or acidified growth environments, further options for process optimization, and novel carrier design strategies based on inhibitor charge expulsion. 2022-01-10T14:33:53Z 2022-01-10T14:33:53Z 2021 Статья Črnivec, I. G. O., Neresyan, T., Gatina, Y., Bučar V. K., Skrt M., Dogša I., Matijašić B. B., Kulikova, I., Lodygin, A., Ulrih, N.P. Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions // Molecules. - 2021. - Том 26. - Выпуск 24. - Номер статьи 7484. - DOI10.3390/molecules26247484 http://hdl.handle.net/20.500.12258/18563 en Molecules application/pdf application/pdf MDPI
institution	СКФУ
collection	Репозиторий
language	English
topic	Dairy products Encapsulation Hydrogels Lactic acid bacteria Lactose-fermenting yeasts Polysaccharides Probiotics
spellingShingle	Dairy products Encapsulation Hydrogels Lactic acid bacteria Lactose-fermenting yeasts Polysaccharides Probiotics Neresyan, T. Нересян, Т. Gatina, Y. S. Гатина, Ю. С. Kulikova, I. K. Куликова, И. К. Lodygin, A. D. Лодыгин, А. Д. Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
description	Adverse environmental conditions are severely limiting the use of microorganisms in food systems, such as probiotic delivery, where low pH causes a rapid decrease in the survival of ingested bacteria, and mixed-culture fermentation, where stepwise changes and/or metabolites of individual microbial groups can hinder overall growth and production. In our study, model probiotic lactic acid bacteria (L. plantarum ATCC 8014, L. rhamnosus GG) and yeasts native to dairy mixed cultures (K. marxianus ZIM 1868) were entrapped in an optimized (cell, alginate and hardening solution concentration, electrostatic working parameters) Ca-alginate system. Encapsulated cultures were examined for short-term survival in the absence of nutrients (lactic acid bacteria) and longterm performance in acidified conditions (yeasts). In particular, the use of encapsulated yeasts in these conditions has not been previously examined. Electrostatic manufacturing allowed for the preparation of well-defined alginate microbeads (180–260 µm diameter), high cell-entrapment (95%) and viability (90%), and uniform distribution of the encapsulated cells throughout the hydrogel matrix. The entrapped L. plantarum maintained improved viabilities during 180 min at pH 2.0 (19% higher when compared to the free culture), whereas, L. rhamnosus appeared to be less robust. The encapsulated K. marxianus exhibited double product yields in lactose-and lactic acid-modified MRS growth media (compared to an unfavorable growth environment for freely suspended cells). Even within a conventional encapsulation system, the pH responsive features of alginate provided superior protection and production of encapsulated yeasts, allowing several applications in lacto-fermented or acidified growth environments, further options for process optimization, and novel carrier design strategies based on inhibitor charge expulsion.
format	Статья
author	Neresyan, T. Нересян, Т. Gatina, Y. S. Гатина, Ю. С. Kulikova, I. K. Куликова, И. К. Lodygin, A. D. Лодыгин, А. Д.
author_facet	Neresyan, T. Нересян, Т. Gatina, Y. S. Гатина, Ю. С. Kulikova, I. K. Куликова, И. К. Lodygin, A. D. Лодыгин, А. Д.
author_sort	Neresyan, T.
title	Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
title_short	Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
title_full	Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
title_fullStr	Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
title_full_unstemmed	Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
title_sort	polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions
publisher	MDPI
publishDate	2022
url	https://dspace.ncfu.ru/handle/20.500.12258/18563
work_keys_str_mv	AT neresyant polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT neresânt polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT gatinays polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT gatinaûs polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT kulikovaik polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT kulikovaik polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT lodyginad polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions AT lodyginad polysaccharidehydrogelsfortheprotectionofdairyrelatedmicroorganismsinadverseenvironmentalconditions
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Polysaccharide hydrogels for the protection of dairy-related microorganisms in adverse environmental conditions

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