CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME

Magnetically guided biodegradable nanocomposites are a new class of functional materials that expand the possibilities of clinical therapy. The paper describes the chemistry of the formation of a nanobiocomposite material based on a natural polymer synthesized by electrostatic interaction of a polys...

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Главные авторы:	Suprunchuk, V. E., Супрунчук, В. Е.
Формат:	Статья
Язык:	Russian
Опубликовано:	2023
Темы:	Fucoidan Nanoparticles Nanobiocomposite Tissue plasminogen activator Magnetite
Online-ссылка:	https://dspace.ncfu.ru/handle/20.500.12258/23742
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id	ir-20.500.12258-23742
record_format	dspace
spelling	ir-20.500.12258-237422025-02-11T07:47:45Z CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME СОЗДАНИЕ И СВОЙСТВА БИОКОМПОЗИТНЫХ НАНОЧАСТИЦ НА ОСНОВЕ ФУКОИДАНА КАК НОСИТЕЛЕЙ ФИБРИНОЛИТИЧЕСКОГО ФЕРМЕНТА Suprunchuk, V. E. Супрунчук, В. Е. Fucoidan Nanoparticles Nanobiocomposite Tissue plasminogen activator Magnetite Magnetically guided biodegradable nanocomposites are a new class of functional materials that expand the possibilities of clinical therapy. The paper describes the chemistry of the formation of a nanobiocomposite material based on a natural polymer synthesized by electrostatic interaction of a polysaccharide and magnetite in an aqueous medium. The possibility of electrostatic immobilization of a model fibrinolytic enzyme was studied. An increase in the electrochemical potential of particles during immobilization of a fibrinolytic enzyme was established by the method of dynamic light scattering. The degree of enzyme inclusion was 9.95±0.05 mass%. The average particle size of the nanobiocomposite material after immobilization of the enzyme was 264.3 nm. The study of the magnetic properties showed the absence of residual magnetization, which makes it possible to exclude the possibility of magnetic aggregation when using the biocomposite in a biological application. The saturation magnetization of the formed material was 57.1 ± 2.3 kA/m, which is lower than that of pure magnetite sol (MS ∼ 73.8 ± 3.4 kA/m), but sufficient for manipulation under conditions of resistance to blood flow. The kinetics of the release of an enzyme preparation in a phosphate buffer from a nanocomposite carrier based on fucoidan was studied. To study the kinetics of enzyme release by mathematical modeling, zero and first order models, the Korsmeier-Peppas model, the Higuchi model, and the Baker-Lonsdale model were used. It has been established that the kinetics of fibrinolytic enzyme release is described with high accuracy (r2 = 0.98) by the Korsmeier–Peppas equation, where the process of release of the enzyme preparation is influenced by diffusion obeying Fick's law. It has been established that zero and first order models are applicable only to the initial (0-40 min, where r2 = 0.96) and final (80-320 min, r2 = 0.93) stage of release. 2023-06-28T14:56:38Z 2023-06-28T14:56:38Z 2023 Статья Suprunchuk, V.E. CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME // ChemChemTech. - 2023. - 66 (5), pp. 87-95. - DOI: 10.6060/ivkkt.20236605.6680 http://hdl.handle.net/20.500.12258/23742 ru ChemChemTech application/pdf application/pdf
institution	СКФУ
collection	Репозиторий
language	Russian
topic	Fucoidan Nanoparticles Nanobiocomposite Tissue plasminogen activator Magnetite
spellingShingle	Fucoidan Nanoparticles Nanobiocomposite Tissue plasminogen activator Magnetite Suprunchuk, V. E. Супрунчук, В. Е. CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME
description	Magnetically guided biodegradable nanocomposites are a new class of functional materials that expand the possibilities of clinical therapy. The paper describes the chemistry of the formation of a nanobiocomposite material based on a natural polymer synthesized by electrostatic interaction of a polysaccharide and magnetite in an aqueous medium. The possibility of electrostatic immobilization of a model fibrinolytic enzyme was studied. An increase in the electrochemical potential of particles during immobilization of a fibrinolytic enzyme was established by the method of dynamic light scattering. The degree of enzyme inclusion was 9.95±0.05 mass%. The average particle size of the nanobiocomposite material after immobilization of the enzyme was 264.3 nm. The study of the magnetic properties showed the absence of residual magnetization, which makes it possible to exclude the possibility of magnetic aggregation when using the biocomposite in a biological application. The saturation magnetization of the formed material was 57.1 ± 2.3 kA/m, which is lower than that of pure magnetite sol (MS ∼ 73.8 ± 3.4 kA/m), but sufficient for manipulation under conditions of resistance to blood flow. The kinetics of the release of an enzyme preparation in a phosphate buffer from a nanocomposite carrier based on fucoidan was studied. To study the kinetics of enzyme release by mathematical modeling, zero and first order models, the Korsmeier-Peppas model, the Higuchi model, and the Baker-Lonsdale model were used. It has been established that the kinetics of fibrinolytic enzyme release is described with high accuracy (r2 = 0.98) by the Korsmeier–Peppas equation, where the process of release of the enzyme preparation is influenced by diffusion obeying Fick's law. It has been established that zero and first order models are applicable only to the initial (0-40 min, where r2 = 0.96) and final (80-320 min, r2 = 0.93) stage of release.
format	Статья
author	Suprunchuk, V. E. Супрунчук, В. Е.
author_facet	Suprunchuk, V. E. Супрунчук, В. Е.
author_sort	Suprunchuk, V. E.
title	CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME
title_short	CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME
title_full	CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME
title_fullStr	CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME
title_full_unstemmed	CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME
title_sort	creation and properties of biocomposite nanoparticles based on fucoidan as carriers of fibrinolytic enzyme
publishDate	2023
url	https://dspace.ncfu.ru/handle/20.500.12258/23742
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CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME

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