Spontaneous and induced platelet aggregation during pregnancy and labor

Objective: to evaluate changes in characteristics of spontaneous platelet (Pt) aggregation in patients with obstetric complications associated with hereditary thrombophilia. Materials and methods. Blood samples were taken from 52 recently confined women on the first day after labor; at that, ethic r...

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Главные авторы:	Bondar, T. P., Бондарь, Т. П., Muratova, A. Y., Муратова, А. Ю., Melchenko, E. A., Мельченко, Е. А.
Formato:	Статья
Idioma:	Russian
Publicado em:	V.A. Negovsky Research Institute of General Reanimatology 2018
Assuntos:	Gene polymorphism Platelet aggregation activity Pregnant women Scanning probe microscopy Thrombophilia
Acesso em linha:	https://www.scopus.com/record/display.uri?eid=2-s2.0-85011000161&origin=resultslist&sort=plf-f&src=s&nlo=1&nlr=20&nls=afprfnm-t&affilName=north+caucasus+federal+university&sid=4a5a00547f1a7b38888040771974d4c5&sot=afnl&sdt=cl&cluster=scopubyr%2c%222016%22%2ct&sl=53&s=%28AF-ID%28%22North+Caucasus+Federal+University%22+60070541%29%29&relpos=104&citeCnt=0&searchTerm= https://dspace.ncfu.ru/handle/20.500.12258/3360
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spelling	ir-20.500.12258-33602020-07-30T13:30:17Z Spontaneous and induced platelet aggregation during pregnancy and labor Bondar, T. P. Бондарь, Т. П. Muratova, A. Y. Муратова, А. Ю. Melchenko, E. A. Мельченко, Е. А. Gene polymorphism Platelet aggregation activity Pregnant women Scanning probe microscopy Thrombophilia Objective: to evaluate changes in characteristics of spontaneous platelet (Pt) aggregation in patients with obstetric complications associated with hereditary thrombophilia. Materials and methods. Blood samples were taken from 52 recently confined women on the first day after labor; at that, ethic regulations for the pre-analytical phase were followed. Determination of PlA1/PlA2 polymorphism enotype was performed by means of amplification-restriction analysis. Geometrical characteristics of patients' peripheral blood Pt aggregation were studied by means of AFM Integra Prima. The degree of confidence of the parameters under test was determined using the t-test, and the significance level was considered valid at P<0.05. Results. A statistical analysis of the findings demonstrated that the length of Pt aggregates in healthy pregnant women was significantly higher than that in healthy non-pregnant women at all study phases. Patients with the P1A1/P1A2 polymorphism in the GP IIb/IIIa Pt receptor gene demonstrated increased widthm height, and density of Pt aggregates. The changes were most significant during the incubation phase lasting for 15 and 30 minutes. The study of geometric parameters of different exposures demonstrated the following: the longer the incubation period, the greater the difference between geometric parameters of the aggregates (e.g. height, length, and width). Conclusion. The analysis of obtained data demonstrated that the presence of P1A1/P1A2 polymorphism in GP IIb/IIIa Pt gene receptor contributes to the decrease in the platelet response threshold and enhances the spontaneous Pt aggregation. The imaging of aggregates provides strong evidence for the accelerated growth of the aggregates in thrombotic complications of pregnancy. © 2016, V.A. Negovsky Research Institute of General Reanimatology. All rights reserved 2018-10-31T08:52:34Z 2018-10-31T08:52:34Z 2016 Статья Bondar, T.P., Muratova, A.Y., Melchenko, E.A. Spontaneous and induced platelet aggregation during pregnancy and labor // Obshchaya Reanimatologiya. - 2016. - Volume 12. - Issue 5. - Pages 16-22 https://www.scopus.com/record/display.uri?eid=2-s2.0-85011000161&origin=resultslist&sort=plf-f&src=s&nlo=1&nlr=20&nls=afprfnm-t&affilName=north+caucasus+federal+university&sid=4a5a00547f1a7b38888040771974d4c5&sot=afnl&sdt=cl&cluster=scopubyr%2c%222016%22%2ct&sl=53&s=%28AF-ID%28%22North+Caucasus+Federal+University%22+60070541%29%29&relpos=104&citeCnt=0&searchTerm= http://hdl.handle.net/20.500.12258/3360 ru Obshchaya Reanimatologiya application/pdf V.A. Negovsky Research Institute of General Reanimatology
institution	СКФУ
collection	Репозиторий
language	Russian
topic	Gene polymorphism Platelet aggregation activity Pregnant women Scanning probe microscopy Thrombophilia
spellingShingle	Gene polymorphism Platelet aggregation activity Pregnant women Scanning probe microscopy Thrombophilia Bondar, T. P. Бондарь, Т. П. Muratova, A. Y. Муратова, А. Ю. Melchenko, E. A. Мельченко, Е. А. Spontaneous and induced platelet aggregation during pregnancy and labor
description	Objective: to evaluate changes in characteristics of spontaneous platelet (Pt) aggregation in patients with obstetric complications associated with hereditary thrombophilia. Materials and methods. Blood samples were taken from 52 recently confined women on the first day after labor; at that, ethic regulations for the pre-analytical phase were followed. Determination of PlA1/PlA2 polymorphism enotype was performed by means of amplification-restriction analysis. Geometrical characteristics of patients' peripheral blood Pt aggregation were studied by means of AFM Integra Prima. The degree of confidence of the parameters under test was determined using the t-test, and the significance level was considered valid at P<0.05. Results. A statistical analysis of the findings demonstrated that the length of Pt aggregates in healthy pregnant women was significantly higher than that in healthy non-pregnant women at all study phases. Patients with the P1A1/P1A2 polymorphism in the GP IIb/IIIa Pt receptor gene demonstrated increased widthm height, and density of Pt aggregates. The changes were most significant during the incubation phase lasting for 15 and 30 minutes. The study of geometric parameters of different exposures demonstrated the following: the longer the incubation period, the greater the difference between geometric parameters of the aggregates (e.g. height, length, and width). Conclusion. The analysis of obtained data demonstrated that the presence of P1A1/P1A2 polymorphism in GP IIb/IIIa Pt gene receptor contributes to the decrease in the platelet response threshold and enhances the spontaneous Pt aggregation. The imaging of aggregates provides strong evidence for the accelerated growth of the aggregates in thrombotic complications of pregnancy. © 2016, V.A. Negovsky Research Institute of General Reanimatology. All rights reserved
format	Статья
author	Bondar, T. P. Бондарь, Т. П. Muratova, A. Y. Муратова, А. Ю. Melchenko, E. A. Мельченко, Е. А.
author_facet	Bondar, T. P. Бондарь, Т. П. Muratova, A. Y. Муратова, А. Ю. Melchenko, E. A. Мельченко, Е. А.
author_sort	Bondar, T. P.
title	Spontaneous and induced platelet aggregation during pregnancy and labor
title_short	Spontaneous and induced platelet aggregation during pregnancy and labor
title_full	Spontaneous and induced platelet aggregation during pregnancy and labor
title_fullStr	Spontaneous and induced platelet aggregation during pregnancy and labor
title_full_unstemmed	Spontaneous and induced platelet aggregation during pregnancy and labor
title_sort	spontaneous and induced platelet aggregation during pregnancy and labor
publisher	V.A. Negovsky Research Institute of General Reanimatology
publishDate	2018
url	https://www.scopus.com/record/display.uri?eid=2-s2.0-85011000161&origin=resultslist&sort=plf-f&src=s&nlo=1&nlr=20&nls=afprfnm-t&affilName=north+caucasus+federal+university&sid=4a5a00547f1a7b38888040771974d4c5&sot=afnl&sdt=cl&cluster=scopubyr%2c%222016%22%2ct&sl=53&s=%28AF-ID%28%22North+Caucasus+Federal+University%22+60070541%29%29&relpos=104&citeCnt=0&searchTerm= https://dspace.ncfu.ru/handle/20.500.12258/3360
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Spontaneous and induced platelet aggregation during pregnancy and labor

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