Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine

Dopamine, adrenaline and octopamine are small polar molecules that play a vital role in regulatory systems. In this paper, phthalylglycyl chloride was proposed as a derivatization agent for octopamine, adrenaline and dopamine determination in urine for the first time. The derivatization procedure fa...

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Главные авторы:	Dotsenko, V. V., Доценко, В. В.
Формат:	Статья
Язык:	English
Опубликовано:	2023
Темы:	Phthalylglycyl chloride Derivatization Catecholamines Dopamine Adrenaline Octopamine
Online-ссылка:	https://dspace.ncfu.ru/handle/20.500.12258/23786
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spelling	ir-20.500.12258-237862025-02-12T07:45:25Z Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine Dotsenko, V. V. Доценко, В. В. Phthalylglycyl chloride Derivatization Catecholamines Dopamine Adrenaline Octopamine Dopamine, adrenaline and octopamine are small polar molecules that play a vital role in regulatory systems. In this paper, phthalylglycyl chloride was proposed as a derivatization agent for octopamine, adrenaline and dopamine determination in urine for the first time. The derivatization procedure facilitated the use of reversed-phase liquid chromatography with positive electrospray ionization–high-resolution mass spectrometry. An LC-HRMS method was developed that provided quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes. The 95–97% yield of derivates was observed after a 10 min derivatization with phthalylglycyl chloride at pH 6.5 and 30 ◦C. The proposed method was successfully applied to the analysis of human urine samples. The obtained results were compared with those of conventional derivatization procedures with 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride. 2023-06-29T11:48:06Z 2023-06-29T11:48:06Z 2023 Статья Zorina, M., Dotsenko, V.V., Nesterenko, P.N., Temerdashev, A., Dmitrieva, E., Feng, Y.-Q., Atapattu, S.N. Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine // Molecules. - 2023. - 28, 2900. - DOI: 10.3390/molecules28072900 http://hdl.handle.net/20.500.12258/23786 en Molecules application/pdf application/pdf
institution	СКФУ
collection	Репозиторий
language	English
topic	Phthalylglycyl chloride Derivatization Catecholamines Dopamine Adrenaline Octopamine
spellingShingle	Phthalylglycyl chloride Derivatization Catecholamines Dopamine Adrenaline Octopamine Dotsenko, V. V. Доценко, В. В. Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
description	Dopamine, adrenaline and octopamine are small polar molecules that play a vital role in regulatory systems. In this paper, phthalylglycyl chloride was proposed as a derivatization agent for octopamine, adrenaline and dopamine determination in urine for the first time. The derivatization procedure facilitated the use of reversed-phase liquid chromatography with positive electrospray ionization–high-resolution mass spectrometry. An LC-HRMS method was developed that provided quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes. The 95–97% yield of derivates was observed after a 10 min derivatization with phthalylglycyl chloride at pH 6.5 and 30 ◦C. The proposed method was successfully applied to the analysis of human urine samples. The obtained results were compared with those of conventional derivatization procedures with 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride.
format	Статья
author	Dotsenko, V. V. Доценко, В. В.
author_facet	Dotsenko, V. V. Доценко, В. В.
author_sort	Dotsenko, V. V.
title	Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
title_short	Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
title_full	Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
title_fullStr	Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
title_full_unstemmed	Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine
title_sort	phthalylglycyl chloride as a derivatization agent for uhplc-ms/ms determination of adrenaline, dopamine and octopamine in urine
publishDate	2023
url	https://dspace.ncfu.ru/handle/20.500.12258/23786
work_keys_str_mv	AT dotsenkovv phthalylglycylchlorideasaderivatizationagentforuhplcmsmsdeterminationofadrenalinedopamineandoctopamineinurine AT docenkovv phthalylglycylchlorideasaderivatizationagentforuhplcmsmsdeterminationofadrenalinedopamineandoctopamineinurine
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Phthalylglycyl Chloride as a Derivatization Agent for UHPLC-MS/MS Determination of Adrenaline, Dopamine and Octopamine in Urine

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